Table 1 PCR primers used to quantify gene expression in Mmtv-Pymt murine mammary tumours. Primers recognise mouse genes
From: Ovarian cycle stage critically affects 21-gene recurrence scores in Mmtv-Pymt mouse mammary tumours
Gene | Primer sequence | Product length (bp) |
|---|---|---|
Mki67 | 5' – AATCCAACTCAAGTAAACGGGG 3' - TTGGCTTGCTTCCATCCTCA | 127 |
Stk15 | 5' – CTGGATGCTGCAAACGGATAG 3' - CGAAGGGAACAGTGGTCTTAACA | 105 |
Birc5 | 5' – CTACCGAGAACGAGCCTGATT 3' - AGCCTTCCAATTCCTTAAAGCAG | 60 |
Ccnb1 | 5' – AGAGCTATCCTCATTGACTGGC 3' - AACATGGCCGTTACACCGAC | 155 |
Mybl2 | 5' – GTGAGGCAGTTTGGACAGCAA 3' - GGATTCAAAACCCTCAGCCA | 101 |
Esr1 | 5' – TGATTGGTCTCGTCTGGCGCT 3' - GCACACAAACTCTTCTCCCTGC | 179 |
Pgr | 5' – CGCCATCTACCAGCCGCTC 3' - TGAATCTGGCCTCAGGTAGTT | 121 |
Bcl2 | 5' – ATGCCTTTGTGGAACTATATGGC 3' - GGTATGCACCCAGAGTGATGC | 120 |
Scube2 | 5' – GGCTGTGTCCACGACTGTTTA 3' - GTTCTCCAAGCATTCGTCCAT | 117 |
Erbb2 | 5' – TGCTCAACTGGTGTGTTCAGATT 3' - TTCGGGCAGCTAGGTCC | 84 |
Grb7 | 5' – ACAAACAGGCATATCCCATGAAG 3' - TAGAGGCCAGATCGACGCA | 159 |
Mmp11 | 5' – GCCTGATGTACTGAATGCCC 3' - GCTCCCTTACAAGCTGCCA | 119 |
Ctsv | 5' – ATCAAACCTTTAGTGCAGAGTGG 3' - CTGTATTCCCCGTTGTGTAGC | 136 |
Gstm1 | 5' – ATACTGGGATACTGGAACGTCC 3' - AGTCAGGGTTGTAACAGAGCAT | 349 |
Cd68 | 5' – TGTCTGATCTTGCTAGGACCG 3' - GAGAGTAACGGCCTTTTTGTGA | 75 |
Bag1 | 5' – GCAGCAGGGAGTTGACTAGAA 3' - TTACTTCCTCGGTTTGGGTCG | 111 |
Actb | 5' – GTGTGACGTTGACATCCGTAAAG 3' - CTCAGGAGGAGCAATGATCTTGAT | 151 |
Gapdh | 5' – ACACATTGGGGGTAGGAACA 3' - AACTTTGGCATTGTGGAAGG | 223 |
Rplp0 | 5' – AGATTCGGGATATGCTGTTGGC 3' - TCGGGTCCTAGACCAGTGTTC | 109 |
Gusb | 5' – CGGGCTGGTGACCTACTGGATT 3' - TGGCACTGGGAACCTGAAGT | 134 |
Tfrc | 5' – GTTTCTGCCAGCCCCTTATTAT 3' - GCAAGGAAAGGATATGCAGCA | 152 |