Fig. 3

Correlation of TDO2 expression with PTEN alteration. a TDO2 expression correlated with mutation of STED2, PTEN and ARID1A genes by TIMER2.0. b qRT-PCR results of TDO2 expression in RCC cell lines. c Western blot analysis of the expression of PTEN and TDO2 in three RCC cell lines. GAPDH served as an internal control. The western blot bands were cropped and full-length blots/gels were presented in Fig. S5, Fig. S6. d, e The levels of TDO2 expression affected by PTEN knockout. d qRT-PCR results of TDO2 expression in PTEN knockout and its parental cells. e Western blot analysis of the expression of PTEN and TDO2 in PTEN knockout and its parental cells. GAPDH served as an internal control. The western blot bands were cropped and full-length blots/gels were presented in Fig. S7, Fig. S8. The bars indicate the mean ± SD (n = 3)