Fig. 4

BTKi inhibited LD accumulation in MCL cells. a SRS imaging analysis of cellular LDs in primary MCL cells (n = 3, primary MCL cells collected from patient 1–3.) treated as the indicated conditions for 24 h. One representative sample is shown. Scale bars, 10 μm. b Lipid area in the fields of view (n = 3) obtained from each sample was quantified based on the SRS images from (a) by ImageJ software. Data are shown as mean ± SEM; *P < 0.05; **P < 0.01, as calculated using the Student’s t-test. c SRS imaging analysis of cellular LDs in JeKo-1 or Mino cells treated as the indicated conditions on the top of the figures. One representative MCL sample is shown. Scale bars, 10 μm. d Lipid area was quantified based on the SRS images from (c) (n = 5) by ImageJ software. Data are shown as mean ± SEM; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001, as calculated using the Student’s t-test