Fig. 4
From: A positive-feedback loop between HBx and ALKBH5 promotes hepatocellular carcinogenesis
HBV up-regulates ALKBH5 via the HBx-WDR5-H3K4me3 axis. A HBx, WDR5 and H3K4me3 distribution at the ALKBH5 gene locus. Snapshot generated by the UCSC Genome Browser. B The H3K4me3 level of ALKBH5 promoter was measured by ChIP-qPCR after either HBx or WDR5 knockdown compared to the control. C WDR5 mRNA TPM score and protein abundance in tumor and paired peri-tumor from 159 Fudan HBV-HCC patients’ transcriptome and proteome dataset. D Left panel: Kaplan-Meier analysis of WDR5 mRNA TPM, protein abundance and overall survival of 159 Fudan HBV-HCC patients. Right panel: Correlation analysis between WDR5 and ALKBH5 mRNA TPM, protein abundance from 159 Fudan HBV-HCC patients’ transcriptome and proteome dataset. E WDR5 protein level in HepG2.2.15 cells with or without ALKBH5 knockdown. T, tumor; P, peri-tumor