Fig. 1

The mRNA and proterin level of ALKBH5 are highly expressed in HBV-HCC tissue. AThe mRNA and B protein levels of ALKBH5 in the indicated HBV-HCC or matched peri-tumor tissues were determined by RT-qPCR (n = 20) and Western blot assays (n = 20), respectively. GAPDH was used as the internal control in RT-qPCR assay and β-actin was used as the normalized control in Western blotting assay. C Representative images of ALKBH5 IHC staining in normal liver, peri-tumor and tumor tissue of HBV-HCC patients (magnification 200×). D ALKBH5 IHC score in tumor and paired peri-tumor from 79 HBV-HCC tissue microarray. E Kaplan-Meier analysis of ALKBH5 IHC score and overall survival from 79 HBV-HCC tissue microarray. T, tumor; P, peri-tumor