Fig. 5
Expression of miR-203a-3p and E-cadherin in vivo. BALB/c-nu/nu female mice, 6- to 8-weeks-of-age, were divided randomly into 2 groups, separately injected LoVo-P / LoVo-C in spleens. (A) Image of tumor in spleen and liver. (B-C) Liver metastasis score and weight of mice. qPCR (D) and western blot assays (E) examining the expression of miR-203a-3p, PRL-3, Src, p-EGFR, p-PKC and E-cadherin in the spleen site and liver metastases. (F) IHC examining the p-EGFR, p-PKC and E-cadherin immunoreactivity in the spleen site and liver metastases. (G) Expression of miR-203a-3p/E-cadherin (E-cad)/Snail in the primary site and liver metastases. (H) Correlation between miR-203a-3p/E-cadherin (E-cad) and Src/p-EGFR expression