Fig. 3

Anchorage-independent growth and invasion. A High-risk HPV16 E6 + EBV LMP-1 (16E6 + LMP-1) formed many colonies on DMEM with agar, whereas low-risk HPV6 E6 + EBV LMP-1 (6E6 + LMP-1) was unable to form any colonies. B The number of colonies formed on DMEM with agar was counted and compared among clones. High-risk HPV16 E6 + EBV LMP-1 (16E6 + LMP-1) formed about 40 colonies of various sizes, but low-risk HPV6 E6 + EBV LMP-1 (6E6 + LMP-1) demonstrated no colony formation. C High-risk HPV16 E6 + EBV LMP-1 (16E6 + LMP-1) displayed higher invasive capacity than other clones, whereas low-risk HPV6 E6 + EBV LMP-1 (6E6 + LMP-1) exhibited only slightly more invasion than mock cells. D The invasive capacity of each clone was quantified in relation to the level of mock cells set to 1.0. High-risk HPV16 E6 + EBV LMP-1 (16E6 + LMP-1) showed a two-fold higher invasive capacity than mock cells. However, low-risk HPV6 E6 + EBV LMP-1 (6E6 + LMP-1) demonstrated a slightly increased invasive capacity compared with the single viral protein expressing clones