Fig. 2

MCM3AP-AS1 might bind to miR-148a, while MCM3AP-AS1 and miR-148a did not affect each other’s expression. The interaction between MCM3AP-AS1 and miR-148a was predicted using IntaRNA (http://rna.informatik.uni-freiburg.de/IntaRNA/Input.jsp). It was observed that miR-148a could form base pairing with MCM3AP-AS1 (A, left). The direct interaction between miR-148a and MCM3AP-AS1 was confirmed by RNA pull-down assay (A, right) and luciferase reporter assay (B). To further analyze the interaction between MCM3AP-AS1 and miR-148a, SHP-77 cells were transfected with MCM3AP-AS1 expression vector or miR-148a mimic. The overexpression of MCM3AP-AS1 and miR-148a was confirmed by qPCR (C). The interaction between MCM3AP-AS1 and miR-148a was analyzed by qPCR (D). The mean values of 3 biological replicates were presented. *, p < 0.05. C: control; NC: negative control; WT: wild type; Mut: mutant type