Fig. 1

Genome-sale CRISPR library knockout screening for genes associated with lapatinib resistance in GC cell lines. a Schematic diagram of the CRISPR library screening strategy. The loss of function screening was performed with the infection of pooled lentivirus containing the GeCKO library V2.0 on N87 and OE19 cells, followed by puromycin selection and lapatinib treatment. The cells were harvested for genomic DNA to PCR the sgRNAs and the subsequent sequencing after 14 days post treatment. b The distribution of sgRNA frequencies in the untreated (baseline), the vehicle-treated (DMSO), and lapatinib-treated N87 and OE19 cells, respectively. c Scatterplot showing identification of top 10 candidate genes using Model-based Analysis of Genome-wide CRISPR-Cas9 Knockout (MAGeCK). Wilcoxon rank-sum test indicated the following, **significant difference from the control, p < 2.2e-16