Table 1 List of primers used for RT-qPCR
Gene | Forward primer sequence 5′ → 3′ | Reverse primer sequence 5′ → 3′ | Amplicon length (bp) |
|---|---|---|---|
TNFaRT | CAGAGGGAAGAGTTCCCCAG | CCTTGGTCTGGTAGGAGACG | 325 |
TNFR1 | ACCAAGTGCCACAAAGGAAC | GTTTTCTGAAGCGGTGAAGG | 105 |
TNFR2 | GCTCTGACCAGGTGGAAACTCAAG | GGATGAAGTCGTGTTGGAGAACG | 224 |
TRAIL | ACCAACGAGCTGAAGCAGAT | ACGGAGTTGCCACTTGACTT | 141 |
IL6 | AAAGAGGCACTGGCAGAAAA | TTTCACCAGGCAAGTCTCCT | 99 |
CD133 | TTGTGGCAAATCACCAGGTA | TCAGATCTGTGAACGCCTTG | 162 |
Nestin | AGCCCTGACCACTCCAGTTTAG | CCCTCTATGGCTGTTTCTTTCTCT | 128 |
OCT4 | ACATCAAAGCTCTGCAGAAAGAACT | CTGAATACCTTCCCAAATAGAACCC | 127 |
SOX2 | GGAAAGTTGGGATCGAACAA | GCGAACCATCTCTGTGGTCT | 145 |
WNT7B | GTCCTGTACGTGAAGCTCGG | GTACTGGCACTCGTTGATGC | 174 |
STAT3 | GCCAGAGAGCCAGGAGCA | GGTTCAGCACCTTCACCATT | 174 |
HPRT1 | GACCAGTCAACAGGGGACAT | CCTGACCAAGGAAAGCAAAG | 132 |