Fig. 6

Cell cycle analysis. a Quantification of cell cycle profile analyzed by flow cytometry in HT29 and HCT116 cell lines after 48 h treatment with 5-FU and/or Olaparib (n = 3). *p < 0.05 above error bars vs. controls at the same cell cycle phase; #p < 0.05 vs. 5⎧M 5-FU in monotherapy. b Representative histograms of cell cycle profile in HT29 and HCT116 cell lines after 48 h treatment with 5-FU and/or Olaparib. c Polyploidy (>4n) population accumulation following 48 h treatment with 5-FU and/or Olaparib Untreated cells were used as negative control (c). *p < 0.05, **p < 0.01 and ***p < 0.001 above error bars vs. controls. Two-way ANOVA and Tukey’s post-hoc tests