Fig. 3

Inhibition of COMPASS complex and NF-kB signaling by MKL1 silencing in HCC cell lines. a The decreases of WRR5, Ash2, SET1 and p65 mRNA levels in HepG2 and Huh-7 cells induced by MKL1 silencing. The mRNA levels of WRR5, Ash2, SET1 and p65 genes were measured by quantitative RT-PCR method, using GAPDH as the internal standard. b The reduced abundances of WRR5, Ash2, SET1 and p65 proteins in HepG2 and Huh-7 cells transfected with siMKL1. WRR5, Ash2, SET1 and p65 protein levels in HCC cells were detected by western blotting, with GAPDH as the internal standard. Full-length blots/gels are presented in Additional files 1C *P < 0.05; **P < 0.01