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Fig. 7 | BMC Cancer

Fig. 7

From: COXIBs and 2,5-dimethylcelecoxib counteract the hyperactivated Wnt/β-catenin pathway and COX-2/PGE2/EP4 signaling in glioblastoma cells

Fig. 7

Analysis of COX-2 and PGE2 EP4 expression in different cell lines. A-172 (a), T98G (b), U-138 MG (c), P1 (d) and P6 (e) cells were treated with celecoxib, 2,5-DMC and rofecoxib for 48 and 72 h. The concentration of compounds used in this assay provided at least 70% of cell viability. PGE EP4 and COX-2 protein levels were evaluated by Western blot. DMSO was used as a control. GAPDH was used as loading control. Graphs represent densitometric analysis of protein of interest to loading control

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