Fig. 2
From: Acquisition of cancer stem cell capacities after spontaneous cell fusion
a. Percentage of ALDHhigh cells in parental and hybrid cell lines evaluated by flow cytometry. * p value < 0,05; n = 4. b. Number of spheres after 10 days of culture in non-adherent condition. n = 6 c. Evaluation of stem cell frequency by ELDA method in spheres formed to evaluate self-renew capacity. d. Expression of NANOG and OCT4 by western blotting analysis in the monolayer culture of each cell lines. β-actin was used as loading control. Full-length blots are presented in Supplementary Fig. 4