Fig. 5
From: FOXD1 regulates cell division in clear cell renal cell carcinoma
G2 progression and histone H3 phosphorylation at ser10 are impaired in FOXD1 null cells. a Cell cycle analysis of synchronized cells at intervals after release from double thymidine block. Red arrows indicate a significant difference (p < 0.05) in phase distribution between lines after 10 h post-block (N = 2). b Western blot analysis of proteins necessary for progression through G2/M at intervals after release from synchronized block, comparing 786-O and 786-OFOXD1null. Predicted cell cycle phases at each time point are graphically represented above the blots. Full-length blots are presented in Supplementary Figs. S5, S6, and S7. c Representative pH3 (green) immunocytochemistry of 786-O parent and 786-OFOXD1null of cells in metaphase, counterstained with phalloidin (blue) and DAPI (gray)