Fig. 3
From: FOXD1 regulates cell division in clear cell renal cell carcinoma
FOXD1 influences mitochondria levels, but does not promote glycolysis. a Western Blot analysis of MICU1 in 786-O versus 786-OFOXD1null. Loading was normalized by total protein in each lane and the full length blot is available in Supplemental Fig. S7. b Immunofluorescence analysis of mitochondria marker TOMM20 (red). Actin filaments (green) used to outline cell body and DAPI (blue) used to outline nucleus (N = 2 biological replicates). c Quantification of TOMM20 fluorescence intensity between 786-O and 786-OFOXD1null using integrated density. * p < 0.05. d Basal mitochondrial respiration measured by Seahorse XF mitochondrial stress test (3 biological replicates). e Representative basal glycolytic rate comparison via Seahorse XF glycolytic rate assay (2 biological replicates). Y axis values are in pmol/min/1000cells. f Representative total ATP production rate comparison via Seahorse XF real-time ATP rate assay (2 biological replicates). Y axis values are in pmol/min/1000cells. For all Seahorse measurements, each biological replicate was measured in 5 technical replicates. *p < 0.05 g Expression of genes involved in mitochondrial respiration in edited versus parent cells. *p < 0.05