Fig. 5

Silencing of GOLIM4 restores cell proliferation and migration and inhibits apoptosis induced by the downregulation of miR-105-3p. Either sh-NC or sh-GOLIM4 was transfected into miR-105-3p-downregulated MCF7 and ZR-75-30 cells, and the cells cotransfected with NC inhibitor and sh-NC were chosen as controls. a RT-qPCR analysis of the expression level of GOLIM4 in MCF-7 and ZR-75-30 cells after transfection with sh-NC or sh-GOLIM4. (B, C) CCK-8 and colony formation assays for cell viability and proliferation. d TUNEL assay for cell apoptosis. e Western blot analysis for related proteins that were involved in cell apoptosis. e Transwell chamber assay for cell migration and invasion. Asterisks indicate significant differences from the control (**P < 0.01, compared to the sh-NC and NC inhibitor+ sh-NC group. ^##P < 0.01, compared to miR-105-3p miRNA inhibitor + sh-GOLIM4)