Mutational profiles of marker genes of cervical carcinoma in Bangladeshi patients

BMC Cancer

Table 2 Primers used for detection of HPV targeting L1 region

Primers	Primer Sequence	Amplicon size (bp)	Annealing temperature (°C)	References
MY11 (forward)	GCMCAGGGWCATAAYAATGGG	450	55	Sin Hang Lee, 2012 [17]
MY09 (reverse)	CGTCCMARRGGAWACTGATC	450	55	Sin Hang Lee, 2012 [17]
GP5 (forward)	TTTGTTACTGTGGTAGATAC	150	49.2	de Roda Husman et al., 1995 [18]
GP6 (reverse)	GAAAAATAAACTGTAAATCA	150	49.2	de Roda Husman et al., 1995 [18]
HPV-16 specific forward (inner)	TACCTACGACATGGGGAGGA	194	55	Ge et al., 2012 [19]
HPV-16 specific reverse (inner)	GCAATTGCCTGGGATGTTAC	194	55	Ge et al., 2012 [19]
HPV-18 specific forward (inner)	TGGTGTTTGCTGGCATAATC	339	55	Ge et al., 2012 [19]
HPV-18 specific reverse (inner)	GCAGCATCCTTTTGACAGGT	339	55	Ge et al., 2012 [19]

Key to degenerate nucleotides: M = (A + C), R = (A + G), W = (A + T), Y = (C + T)

ISSN: 1471-2407