Fig. 4

WNT7b was associated with epithelial to mesenchymal transition in CRC cells. a and d Western blot analysis detecting epithelial to mesenchymal transition marker E-Cadherin and Vimentin protein expressed in normal colorectal cells (FHC) and colorectal cancer cells (HCT116). b and e WNT7b was knocked down by transiently transfection of short interfering RNA of HCT116 cells. c and f The Wnt signaling pathway was inhibited by its antagonist Dickkopf-1 (DKK1). g Trans-well assay analyses of the migratory capacity changes as WNT7b was partially knocked down in HCT116 cells. Bars, 500 μm. h Quantification of migrated cells of each group based on three randomly chosen fields, χ² test