Fig. 3

Glutamine starvation reduces the GSH level and CDDP resistance in A2780cis cells. a Levels of Gln, Glu, and GSH in A2780 and A2780cis cells cultured in the presence (+) or absence (−) of glutamine. b Effects of glutamine starvation on CDDP resistance. A2780 and A2780cis cells were treated with 3 μM CDDP in the presence (+) or absence (−) of glutamine for 48 h. c A2780 and A2780cis cells were cultured for 48 h in medium containing CDDP (0 or 10 μM) and the GLS inhibitor compound 968 (0, 1, 3, or 10 μM). Cell viability was measured using the MTT assay. Data are shown as the mean ± SD of the three independent experiments. The differences were analyzed by using the Student’s t-test (*p < 0.05, **p < 0.01, ***p < 0.001)