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Table 2 Top 50 DEG, 29 upregulated and 21 downregulated, observed exclusively in all the ten samples of adenocarcinoma (presented by FC)

From: ETV4 plays a role on the primary events during the adenoma-adenocarcinoma progression in colorectal cancer

Gene

RefSeq

Description

FCa

FDRb

Upregulated

SIM2

NM_005069.3

single-minded family bHLH transcription factor 2

4.26

7.63E-07

FAP

NM_001291807.1

fibroblast activation protein, alpha

3.34

0.00112

FUT1

NM_000148.3

fucosyltransferase 1

3.04

0.00017

ESM1

NM_001135604.1

endothelial cell-specific molecule 1

2.98

0.00060

FABP6

NM_001040442.1

fatty acid binding protein 6, ileal

2.95

0.00420

COL1A1

NM_000088.3

collagen, type I, alpha 1

2.75

0.00181

WNT2

NM_003391.2

wingless-type MMTV integration site family member 2

2.65

0.00601

ELN

NM_000501.3

elastin

2.22

0.00072

ETV4

NM_001079675.2

ets variant 4

2.18

0.00060

ACAN

NM_001135.3

aggrecan

2.12

0.00107

LARP6

NM_001286679.1

La ribonucleoprotein domain family, member 6

2.00

0.00111

FADS1

NM_013402.4

fatty acid desaturase

2.00

0.00099

SCRN1

NM_001145513.1

secernin 1

1.97

0.00188

LY6E

NM_001127213.1

lymphocyte antigen 6 complex, locus E

1.95

0.00691

TCFL5

NM_006602.2

transcription factor-like 5 (basic helix-loop-helix)

1.91

0.00426

PROCR

NM_006404.4

protein C receptor, endothelial

1.90

0.00745

FAM150A

NM_001195732.1

family with sequence similarity 150 member A

1.87

0.00283

COL5A2

NM_000393.3

collagen, type V, alpha 2

1.73

0.00601

FAM72D

NM_207418.2

family with sequence similarity 72 member D

1.62

0.00262

SPARC

NM_003118.3

secreted protein, acidic, cysteine-rich (osteonectin)

1.62

0.00420

PARVB

NM_001003828.2

parvin, beta

1.56

0.01610

VEGFA

NM_001025366.2

vascular endothelial growth factor A

1.46

0.00123

UBE2C

NM_001281741.1

ubiquitin-conjugating enzyme

1.45

0.00060

AUNIP

NM_001287490.1

aurora kinase A and ninein interacting protein

1.35

0.00177

GK

NM_000167.5

glycerol kinase

1.26

0.00437

LRP8

NM_001018054.2

low density lipoprotein receptor-related protein 8, apolipoprotein e receptor

1.15

0.01137

DIAPH3

NM_001042517.1

diaphanous-related formin 3

1.11

0.00178

FAM162B

NM_001085480.2

family with sequence similarity 162 member B

1.05

0.04851

CENPJ

NM_018451.4

centromere protein J

1.04

0.00243

Downregulated

CLCA1

NM_001285.3

chloride channel accessory 1

−7.97

0.00155

HEPACAM2

NM_001039372.2

HEPACAM family member 2

−4.05

0.00131

B3GNT6

NM_138706.4

UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 6

−4.03

0.00177

BEST2

NM_017682.2

bestrophin 2

−3.86

0.00099

L1TD1

NM_001164835.1

LINE-1 type transposase domain containing 1

−3.52

0.02738

ADH1A

NM_000667.3

alcohol dehydrogenase 1A (class I) alpha polypeptide

−3.48

0.00852

ADH1C

NM_000669.4

alcohol dehydrogenase 1C (class I), gamma polypeptide

−3.39

0.00776

GALNT8

NM_017417.1

polypeptide N-acetylgalactosaminyltransferase 8

−3.24

0.00084

SPTLC3

NM_018327.2

serine palmitoyltransferase, long chain base subunit 3

−2.99

0.00244

SPINK2

NM_001271718.1

serine peptidase inhibitor, Kazal type 2 (acrosin-trypsin inhibitor)

−2.49

0.01166

ATOH8

NM_153778.3

atonal homolog 8

−2.32

0.00643

KCNMA1

NM_001014797.2

potassium large conductance calcium-activated channel, subfamily M, alpha member 1

−2.27

0.00060

NEURL

NM_004210.4

neuralized E3 ubiquitin protein ligase 1

−2.19

0.00109

PIGR

NM_002644.3

polymeric immunoglobulin receptor

−2.14

0.01272

MFSD4

NM_181644.4

major facilitator superfamily domain containing 4

−2.11

0.00201

ARHGAP44

NM_014859.4

Rho GTPase activating protein 44

−2.06

0.00060

SERTAD4

NM_019605.3

SERTA domain containing 4

−1.82

0.00072

PLA2G10

NM_003561.1

phospholipase A2, group X

−1.73

0.04438

NEDD4L

NM_001144964.1

eural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase

−1.61

0.00123

ACADVL

NM_000018.3

acyl-CoA dehydrogenase, very long chain

−1.20

0.00099

PID1

NM_001100818.1

phosphotyrosine interaction domain containing 1

−1.19

0.01212

  1. aFC (log2 fold change); bFDR (false discovery rate). The genes in bold are the same ones selected in Table 1 for validation by RT-qPCR