Fig. 6

mtPC3-CM induced osteoclastogenesis via RAGE. a Control (siCon) or RAGE siRNA (siRAGE) transfected BMMs were subjected to Western blotting to assess protein levels of RAGE and β-actin. b pOCs transfected with siCon or siRAGE were treated with 30% mtPC3-C-CM or mtPC3-S-CM for osteoclastogenesis. Representative TRAP-stained images (left panels) and quantification of TRAP+ MNCs (right panel). Scale bar = 100 μm. ***p < 0.001 by one-way ANOVA with post hoc Tukey’s test. c pOCs transfected with siCon or siRAGE were cultured on dentin slices with 30% mtPC3-CM. Representative confocal images of dentine surfaces (left panels) and values of the depth of pits and resorbed area (right panels) are presented. Scale bar = 100 μm. ***p < 0.001 by unpaired two-tailed Student’s t-test. d pOCs transfected with siCon or siRAGE were treated with 30% mtPC3-CM for 24 h and were then subjected to western blotting to assess the protein levels of RAGE, c-Fos, NFATc1, and β-actin. Data are presented as the mean ± SD. The full-length gels are presented in Supplementary Fig. 3 h and 3i