Fig. 4

S100A4 promotes EMT and cell migration of bone-metastatic prostate cancer cells. a, mtPC3-C or mtPC3-S cells were cultured in the presence of BSA, S100A4 (2 μg/ml), control antibody, or 4A (30 μg/ml) for 48 h, and then protein levels of E-cadherin, β-catenin, N-cadherin, and β-actin were determined by western blotting with the indicated antibodies. b The protein levels of E-cadherin, β-catenin and N-cadherin were analyzed by western blotting with lysates from LNCaP and PC3 cells cultured in the presence of BSA or S100A4 (2 μg/ml) for 48 h. c Cell migration of mtPC3-C or mtPC3-S cells was measured by transwell assay in the presence of BSA or S100A4 (2 μg/ml). Representative images (left panels) and quantification of migrated cells (right panel). Scale bar = 100 μm. d Cell migration of mtPC3-C or mtPC3-S cells was measured by transwell assay in the presence of control antibody or 4A (30 μg/ml). Representative images (left panels) and quantification of migrated cells (right panel). Scale bar = 100 μm. *p < 0.05 and ***p < 0.001 by one-way ANOVA with post hoc Tukey’s test. Data are presented as the mean ± SD. The full-length gels are presented in Supplementary Fig. 3e and 3 f