Fig. 4

MT1JP bound to miR-18a-5p as a sponge and regulated the expression of FBP1. A. The sequence of MT1JP was bound by miR-18a-5p at 16–29 bp and 115–127 bp (miR-18a-5p seed sequences were shown in red, and the mutant sequences were shown in green). B. Dual-luciferase assay was performed to verify the binding of miR-18a-5p to MT1JP. C. The expression level of miR-18a-5p was detected by real-time PCR after overexpression or knockdown of MT1JP. D. The 3’UTR of FBP1 was bound by miR-18a-5p at 78–86 bp (miR-18a-5p seed sequences were shown in red, and the mutant sequences were shown in green). E. Dual-luciferase assay was carried out to confirm the binding of miR-18a-5p to FBP1 3’UTR. F. The expression level of FBP1 was determined in HCCC-9810 and HUCCT1 cells after transfection of miR-18a-5p mimics. (The western blot bands were cropped from Fig. S9 and S10.) G. The expression level of FBP1 after ectopic expression of MT1JP or/and miR-18a-5p. (The western blot bands were cropped from Fig. S11.) H. Immunofluorescent staining was used to detect the expression and distribution of FBP1 after ectopic expression or silencing of MT1JP. (the scale bar represented as 50 μm) (*p < 0.05, **p < 0.01, ***p < 0.001, compared with pcDNA3.1, siRNA NC, or mimics NC)