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Table 1 Primer sequences for RT-qPCR

From: MicroRNA-545 suppresses progression of ovarian cancer through mediating PLK1 expression by a direct binding and an indirect regulation involving KDM4B-mediated demethylation

Gene

Primer sequence (5′-3′)

miR-545

F: CGACAAGGGTCAGCAAACATT

R: GCAGGGTCCGAGGTATTC

Bax

F: TCAGGATGCGTCCACCAAGAAG

R: TGTGTCCACGGCGGCAATCATC

Bcl-2

F: ATCGCCCTGTGGAGAACTACTGAGT

R: GCCAGGAGAAATCAAACAGAGGC

KDM4B

F: GCCGAGAGGAAGTTCAACGCAG

R: TGCCTCCTTCTCAGAGTGTGTAGG

PLK1

F: GCACAGTGTCAATGCCTCCAAG

R: GCCGTACTTGTCCGAATAGTCC

U6

F: CTCGCTTCGGCAGCACA

R: AACGCTTCACGCATTTGC

GAPDH

F: GTCTCCTCTGACTTCAACAGCG

R: ACCACCCTGTTGCTGTAGCCAA

  1. Note: RT-qPCR reverse transcription quantitative polymerase chain reaction, Bcl-2 B-cell lymphoma-2, Bax Bcl-2-associated X, KDM4B Lysine (K)-specific demethylase 4B, PLK1 Polo-like kinase 1, GAPDH glyceraldehyde-3-phosphate dehydrogenase, F forward, R reverse
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BMC Cancer

ISSN: 1471-2407

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