Fig. 4

Change in the levels of downstream and upstream proteins of the mTOR pathway under temsirolimus treatment in four cancer cell lines. There was no difference in the levels of Akt, phospho-Akt [Ser473], and pan-actin among the four cell lines. Phospho-p70 S6 kinase production was lowered by temsirolimus treatment in each cell line. Temsirolimus treatment increased phospho-TSC2 [Thr462] and decreased phospho-4E-BP1 [Ser65 and Thr37/46] levels in the HR-HPV-positive UM-SCC47 cell line. The original, uncropped blots are provided in Additional file 2