Fig. 2

USP44 overexpression inhibits proliferation of 786-O cells and Caki-1 cells. a, c mRNA expression of USP44 in control (ctrl) and overexpression (OE) groups of 786-O cells (a) and Caki-1 cells (c). b, d Protein expression of FLAG in ctrl and OE groups of 786-O cells (b) and Caki-1 cells (d). The recombinant FLAG-USP44 fusion protein was constructed, so detection of FLAG expression reflected USP44 expression. (cropping of blots). e, f Relative proliferation of ctrl and OE groups of 786-O cells (e) and Caki-1 cells (f) in the CCK8 assay. g, h Absorbance at 370 nm in ctrl and OE groups of 786-O cells (g) and Caki-1 cells (h) in the BrdU experiment. i, k mRNA expression of P21 and cyclin D1 in ctrl and OE groups of 786-O cells (i) and Caki-1 cells (k). j, l Protein expression of P21 and cyclin D1 in ctrl and OE groups of 786-O cells (j) and Caki-1 cells (l). (cropping of blots). *P < 0.05, **P < 0.01 vs. the ctrl group. Data are the mean ± SD