Table 1 Oligonucleotides used in this study
Primer set name | Reverse transcripatase reation primer | Real-time quantitative PCR primer | Tm (°C) | Length (bp) |
|---|---|---|---|---|
U6 | 5’CGCTTCACGAATTTGCGTGTCAT3’ | Forward:5’GCTTCGGCAGCACATATACTAAAAT3′ Reverse:5’CGCTTCACGAATTTGCGTGTCAT3’ | 60 | 89 |
miR-16 | 5’GGCGTAGGCAGTGCAGGGTCCGAGGTCTGCCTACGCCCGCCAATA3’ | 16-F:TCGGCTAGCAGCACGT; 16-R:TGATTGCAGGGTCCGAG | 60 | 60 |
miR-29a | 5’GCGTGGTCGGTAACTCGGACCCTTCTACCGACCACGCTAACCGA 3’ | 29a-F:GAACCCCTTAGCACCATCT; 29a/b-R:AGCGTAACTCGGACCCTT | 60 | 66 |
miR-29b | 5’GCGTGGTCGGTAACTCGGACCCTTCTACCGACCACGCAACACTG3’ | 29b-F:GAACCCCTTAGCACCATTT; 29a/b-R:AGCGTAACTCGGACCCTT | 60 | 66 |
miR-26b | 5’GCCGTGACCGTCAGTGGAGGCAAGCCAGACGGTCACGGCACCTAT 3’ | 26b-F:ACGACGGTTTCAAGTAATTCA; 26b-R: TCTCGTCAGTGGAGGCAA | 60 | 65 |
miR-125b | 5’GCCGTACCGTCAGTGGAGGCAAGCCAGACGGTACGGCTCACAAGT 3’ | 125b-F:CTGGACTCCCTGAGACCCT; 125b-R:ATCCGTCAGTGGAGGCA | 60 | 66 |
miR-143 | 5’GGCGTCAGCCAGAGTGGAGGCAAGCCACTGGCTGACGCCGAGCTAC 3’ | 143-F:GCCAGTCCTGAGATGAAGCAC; 143-R:TGAAGAGTGGAGGCAAGC | 60 | 62 |