Fig. 1

Two-way hierarchical clustering of miRNAs and samples. a Data from each miRNA were median centered. Samples are in columns and miRNAs in rows. The color scale shown at the top illustrates the relative expression level of miRNA in the certain slide: red color represents a higher expression level than control sample; green color represents a lower expression level than the control sample.The actual log2(Hy5/Hy3) ratios for the miRNAs are shown in the expression matrix sheet in slide1~slide6 Data File. b Box plot for scale normalization. The box plot shows the miRNA expression profiling before, after and between slide Scale normalization.(top:only within lowest normalization; down:both within and between slide normalization). The main purpose of scale normalization is to control between slide variability. Y-axis represents the log2Ratio M = log2(Hy5/Hy3)