Fig. 6

TNF mRNA is increased in CRC patients and ELN recombinant protein increases TNF protein from bone marrow derived macrophages (BMDM) after LPS challenge. aTNF gene expression was analyzed from colon tissues from healthy controls (n = 5) and CRC patients (n = 31) based on a GSE128449 dataset. bTNF gene expression was analyzed from colon tissues from tumor and adjacent non-tumor tissues from the same CRC patients (n = 17) according to a GSE110224 dataset. c BMDM were isolated from mice and the purity of macrophages were assessed by flow cytometry. d BMDM were cultured on plates coated with recombinant ELN protein, and control plates were coated with PBS only. BMDM cell lysates were collected 24 and 48 h after LPS challenge, and TNF proteins measured by ELISA. n = 6. Results are mean ± SEM. **P < 0.01, ***P < 0.001 compared to control plates. #P < 0.05 compared to plates coated with LPS only