Table 3 Condition of immunohistochemical stains and the immunoreactivity
IHC markers | SOX2 (Nu) | SOX2 (Cyto) | CD24 | Oct4 | IGF-1 | FGFR | VEGF | CD44v6 | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Vendor | Cell Marque (SP76) | Thermo Scientific | abcam | abcam | abcam | Quartett Immunodiagnostika | Invitrogen | |||||||||
Dilution | 1:100 | 1:50 | 1:100 | 1:100 | 1:100 | 1:50 | 1:200 | |||||||||
Positive control | Normal lung alveoli and squamous cell carcinoma | Testicular seminoma | Normal liver | Normal umbilical cord | Blood vessel | Benign urothelial epithelium | ||||||||||
No. | (%) | No. | (%) | No. | (%) | No. | (%) | No. | (%) | No. | (%) | No. | (%) | No. | (%) | |
0 | 13 | (10.3) | 66 | (52.4) | 6 | (4.8) | 0 | (0.0) | 13 | (10.3) | 0 | (0.0) | 0 | (0.0) | 14 | (11.1) |
1+ | 66 | (52.4) | 56 | (44.4) | 79 | (62.7) | 26 | (20.6) | 74 | (58.7) | 7 | (5.6) | 18 | (14.6) | 41 | (32.5) |
2+ | 45 | (35.7) | 4 | (3.2) | 37 | (29.4) | 73 | (57.9) | 33 | (26.2) | 52 | (41.3) | 78 | (63.4) | 43 | (34.1) |
3+ | 2 | (1.6) | 0 | (0.0) | 4 | (3.2) | 27 | (21.4) | 6 | (4.8) | 67 | (53.2) | 27 | (22.0) | 28 | (22.2) |
Total | 126 | (100) | 126 | (100) | 126 | (100) | 126 | (100) | 126 | (100) | 126 | (100) | 123 | (100) | 126 | (100) |