Fig. 3

AFAP1-AS1 absorbs miR-653-5p to regulate RAI14. a Venn diagram (data predicted by microT, miRanda and TargetScan bioinformatics tools) showed 3 probable genes that may interact with miR-653-5p. b qRT-PCR assay examined the expression of 3 mRNAs in miR-653-5p mimics-transfected cells. c qRT-PCR and western blot assays detected the expression of RAI14 in melanoma cells. d qRT-PCR and western blot assays unveiled the expression of RAI14 after overexpressing miR-653-5p or suppressing AFAP1-AS1. e The binding sites between RAI14 and miR-653-5p. f Luciferase reporter assay assessed the binding ability among AFAP1-AS1, miR-653-5p and RAI14. g RIP assay studied the interaction among AFAP1-AS1, miR-653-5p and RAI14. h The interference efficiency of RAI14 was evaluated by qRT-PCR. i The images of the tumors injected with sh-NC or sh-RAI14#1. j The volume of tumors was recorded. k The weight of tumors was measured. l qRT-PCR detected RAI14 mRNA level in the tumor xenografts. m Western blot assay examined Ki67, E-cadherin and N-cadherin protein expressions in sh-NC group or sh-RAI14#1 group in A375 cells collected from tumors. ^*P < 0.05, ^**P < 0.01