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Fig. 1 | BMC Cancer

Fig. 1

From: Long non-coding RNA AFAP1-AS1 accelerates the progression of melanoma by targeting miR-653-5p/RAI14 axis

Fig. 1

AFAP1-AS1 is highly expressed in melanoma cell lines and enhances melanoma tumorigenesis and development. a qRT-PCR assay was conducted to detect the expression of AFAP1-AS1 in melanoma cells and in normal human epidermal melanocyte HEMa-LP cells. b qRT-PCR assay evaluated the knockdown efficiency of sh-AFAP1-AS1#1/2. c-d CCK-8 and colony formation assay reflected cell proliferation. e-f Transwell assays studied cell migration and invasion. g Western blot assay was applied to measure the expression of EMT process related proteins (E-cadherin and N-cadherin) in A375 and M21 cells. **P < 0.01

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BMC Cancer

ISSN: 1471-2407

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