Fig. 2

Expression of three FLT1 isoforms is inhibited in choriocarcinoma cells. a The mRNA expression levels of all FLT1 transcript variants (total-FLT1) in several cells were measured by qRT-PCR using β-actin mRNA as a reference. Results are expressed as a percentage relative to cytotrophoblasts. b The mRNA expression levels of three FLT1 splice variants in cytotrophoblasts and syncytiotrophoblasts. Results are expressed as a ratio relative to the expression of β-actin mRNA. c Immunoprecipitation of FLT1 isoforms from the cell lysates. The immunoprecipitates and cell lysates (input) were subjected to Western blotting. β-actin was used as a loading control. Numbers show the amount of protein subjected to immunoprecipitation. The indicated percentage of cell lysate was used as the input. d Western blotting of sFLT1 proteins secreted into conditioned media. Numbers show the volume of media subjected to heparin-affinity pull-down. Uncropped images of Western blots are presented in Additional file 1: Figure S6. All values represent the mean ± SD (n = 3)