Fig. 4

ctDNA monitoring. Patient-specific ctDNA profiles from cohort B (serial plasma monitoring) characterized by NGS and confirmed by ddPCR. The levels of ctDNA varies according to the clinical course throughout the treatment. Black and red lines denote corresponding cfDNA mutations detected by NGS and ddPCR, respectively. Gray columns denote tumor burden in millimeters, and has been calculated according to RECIST criteria. Specific treatment, treatment modifications, and dose, are provided below each graph. ctDNA was detected in patients 11, 16, 17 and 18 by both NGS and ddPCR (A-D), and only by ddPCR in patients 12, 13 and 14 (E-G). Two patients were NGS and ddPCR silent and are not shown in this Fig. BL, baseline; PD, progression disease; SD, stable disease; PR, partial response; CR, complete response; SU, sunitinib; RE, regorafenib