Fig. 4

Histopathological images of the paired pre- and post-vaccination. a Histopathological analysis of VEGF-A, VEGFR1, VEGFR2, CD34 and PDGFR-β expressions in the tumor of pre- and post- vaccination. A square means tumor cells with positive VEGFR1 or VEGFR2 staining (T: tumor cell, V: vessel; original magnification, × 40; magnification bar, 100 μm). b Immunofluorescent analysis of VEGFR1, VEGFR2 and PDGFR-β expressions in tumor vessels in pre- and post- vaccination (original magnification, × 40; magnification bar, 100 μm). c Histopathological analysis of cleaved caspase3 expression on the tumor vessel of pre- and post- vaccination. (V: vessel; original magnification, × 40; magnification bar, 100 μm). d Immunofluorescent analysis of CD34, VEGFR1, and cleaved caspase3 expressions in the tumor of pre- and post- vaccination. Expression of cleaved caspase 3 was detected on the endothelial cells with slight VEGFR1 expression or without VEGFR1 expression (original magnification, × 40; magnification bar, 100 μm). e Histopathological analysis of Foxp3 expression in the tumor of pre- and post- vaccination (original magnification, × 40; magnification bar, 100 μm; black arrows, positive cells). f Immunofluorescent analysis of Foxp3 and cleaved caspase3 expressions in the tumor of pre- and post- vaccination (original magnification, × 40; magnification bar, 100 μm; white arrow, positive cells). g Relative gene expression of VEGF-A, VEGFR1, VEGFR2 and Foxp3 in the tumors of pre- and post- vaccination