Fig. 3
BRAFV600E CRC cells respond to different doses of the pan-ErbB-family inhibitor afatinib. Cells were exposed to log10-escalating doses of afatinib (AFA-final concentration range: 0.01 μM to 10 μM) for 48 h, harvested, and assayed for (a, c, e) cell viability and (b, d, f) cell death, as described in Fig. 1. Results shown are means ± SD of three independent experiments. Statistics (two-way ANOVA for multiple comparisons): *p < 0.05, **p < 0.01 vs. CTRL (untreated cells)