Fig. 6

a. Panels 1 and 2 refer to the resulting Ct plots (from ViiA7 run) for multiplex detection of the CpG marker m_GRK7, using as template 30 copies of bisulfite-converted and fragmented genomic DNA from breast cancer cell line (MCF7 or MDA-MB-134-VI) mixed with 3000 copies of genomic DNA from human blood (Roche human genomic DNA). Panel 3 refers to negative control, with just the normal blood genomic DNA as a template. b. A digital PCR readout (using Formulatrix Constellation dPCR System) for similar experiments depicted in A. NTC refers to “No template control”. c. The fraction of methylation at a specific CpG site for m_GRK7 CpG site in the genomes of MCF7 and MDA-MB-134-VI cell lines. This information was extracted from Illumina 450 K array-generated datasets deposited in Gene Expression Omnibus (GEO). * Average value extracted from datasets GSE57342, GSE68379, GSE78875, and GSE94943. **Value extracted from dataset GSE68379