Fig. 5

AGR2 expression modulates gemcitabine sensitivity of PANC-1 cells. a Indicative PANC-1 cells were treated with 20 μM gemcitabine (Gem) for 72 h, stained with Annexin V and PI, and then analyzed with flow cytometry. The results were analyzed with FlowJo software. *, p < 0.01 vs. Vector; #, p < 0.05 vs. Vector. b PANC-1 cells stably expressing the Vector or AGR2 were treated with 20 μM of gemcitabine (Gem) for the indicated periods of time. Whole cell lysates were collected and subjected to Western blotting against Caspase-3 and PARP1 with GAPDH as loading control