Fig. 5

Identification of EHHADH mRNA as a target regulated by miR-486-5p in CDDP-R-BC cell lines. a A Venn diagram of mRNA sequences and in silico analyses indicated that 7 putative candidate target genes of miRNA-486-5p were key molecules in cisplatin-resistant BCs. b The EHHADH mRNA expression levels determined by qRT-PCR and (c) its protein levels determined by Western blot in miR-486-5p transfectants compared with mock or miR-control transfectants. *, P < 0.001. The full-length blots/gels are presented in Supplementary Figure 8. d Dual-luciferase reporter assays using vectors encoding putative miRNA target sites for WT or deleted regions. Luminescence intensity was significantly reduced by co-transfection with miR-486-5p and the vector carrying the WT 3′-UTR. *, P < 0.001. e The protein levels of EHHADH in CDDP-R BC and parental BC cells. The full-length blots/gels are presented in Supplementary Figure 8. f EHHADH mRNA expression in the BLCA cohort in TCGA, EHHADH expression in BLCA samples compared with those in normal samples. g EHHADH expression in cisplatin-sensitive (CR and PR) and cisplatin-resistant (SD and PD) groups treated with cisplatin-based chemotherapy. EHHADH expression in patients with or without tumor