Fig. 4

Transfection of miRNA-486-5p improves sensitivity to CDDP in CDDP-R-BC cell lines. The IC50 concentration of cisplatin was given to parental and cisplatin-resistant cells. Transfection of 10 nM miRNA-486-5p increased the cisplatin sensitivity in (a) CDDP-R-BOY and (b) CDDP-R-T24 cells as determined by XTT assay. *, P < 0.001. miRNA-486-5p transfection and cisplatin administration (IC50 dose) to (c) CDDP-R-BOY and (d) CDDP-R-T24 cells clearly had an additive effect and significantly suppressed cell proliferation. *, P < 0.001. e, f Apoptosis assays were carried out using flow cytometric analysis of CDDP-R-BC cells. *, P < 0.001