Fig. 2

Combination of radotinib and Ara-C increases Annexin V-positive HL60 and HEL92.1.7 cells. Cells were treated with 5 μM radotinib and/or 50 nM Ara-C for 48 h. Cells were stained with annexin V-FITC followed by flow cytometric analysis. a Annexin V staining of HL60 cells. b Data show the percentage of Annexin V-positive cells (apoptotic cells) in (a). c Data show the percentage of Annexin V-positive HEL92.1.7 cells. Representative data are shown for at least three independent experiments. These data represent the means ± SEM. Significantly different from control (*) or combination of radotinib and Ara-C (#); *: P < 0.05; **: P < 0.01; ***, ###: P < 0.001. R, radotinib; A, Ara-C; R + A, combination of radotinib and Ara-C