Fig. 1

Afatinib decreases the size of the epidermis and increases skin differentiation markers. Micro-epidermises were treated with afatinib at 3, 10 and 30 nM. Drugs and concentrations effect on microepidermis were assessed with different parameters (a) Micro-epidermis volume incubated with afatinib 30 nM. b actin expression intensity, c microepidermis volume, d desmoglein-1 expression, e Involucrin expression and f filaggrin expression. * p < 0.05, ** p < 0.01, *** p < 0.001