Fig. 5

Knock-down of hsa_circRNA_103809 aggravated the inhibiting effects of cisplatin on cell growth in CR-NSCLC cells in vivo. The mice tumor tissues were collected, and Western Blot analysis was performed to examine the expression levels of Cyclin D1, CDK2, cleaved Caspase-3 and Bax in (a, b) A549/DDP cells (full-length blots/gels are presented in Supplementary Figure S6A-B), (c, d) H1299/DDP cells (full-length blots/gels are presented in Supplementary Figure S6C-D) and (e, f) Calu-3/DDP cells (full-length blots/gels are presented in Supplementary Figure S6E-F). g IHC was performed to examine the expressions and localization of Ki67 protein in mice tumor tissues, the signal intensity in different groups were assessed as follows: Control (+++), Cisplatin (+++), KD-circRNA (+++) and Cis + KD-circRNA (+). (Note: “Control: without vectors transfection and cisplatin treatment”). Each experiment was repeated at least 3 times. *P < 0.05