Fig. 2

The regulatory link between hsa_circRNA_103809, miR-377-3p and GOT1. The online miRDB software (http://mirdb.org/) indicated that miR-377-3p potentially bound to (a) hsa_circRNA_103809 and (F) 3′ untranslated region (3’UTR) of GOT1 mRNA. Dual-luciferase reporter gene system assay was performed to validate the binding sites of miR-377-3p with (b-d) hsa_circRNA_103809 and (G-I) 3′ UTR region of GOT1 mRNA in NSCLC cells. RNA pull-down assay suggested that miR-377-3p tended to be enriched by the biotin-labelled probes for (e) hsa_circRNA_103809 and (j) GOT1 mRNA. (k, l) GOT1 was positively regulated by hsa_circRNA_103809 in NSCLC cells, full-length blots/gels are presented in Supplementary Figure S5A. (m, n) MiR-377-3p inhibited GOT1 expressions in NSCLC cells, full-length blots/gels are presented in Supplementary Figure S5B. (o, p) Hsa_circRNA_103809 sponged miR-377-3p to upregulate GOT1 in NSCLC cells, full-length blots/gels are presented in Supplementary Figure S5C. (Note: “Control: without vectors transfection”). Each experiment was repeated at least 3 times. *P < 0.05