Fig. 1

Representative immunohistochemistry illustrations of MGMT, GLUT2, and Ki-67. 1-1 Representative illustrations before and after digital image analyses of Ki-67 LI. We analyzed nuclear immunoreactivity according to the gradients of brown color (3,3-diaminobenzidine [DAB]) spectrum immunointensity. Tumor cells with blue nuclei were negative, whereas those with yellow, orange, and red nuclei were positive for Ki-67 immunoreactivity. The arrow indicated intratumoral vessels excluded. 1-2 Representative illustrations before and after digital image analyses of MGMT immunoreactivity. MGMT was analyzed only in nuclei according to the gradients of brown color (3,3-diaminobenzidine [DAB]) spectrum intensity. The relative immunointensity of the gradient was evaluated as follows: 0, negative (blue); + 1, weak (yellow); + 2, moderate (orange); + 3, strong (red). 1-3 MGMT and GLUT2 scores