Fig. 5

Tumor growth is suppressed and delayed following long-term combination treatment with CKD-516 and IR. a Quantification of tumor volume and (b) body weight after long-term treatment monotherapy with IR and CKD-516. H520 cells were injected subcutaneously into the right forearm of nude mice. Depending on the administration method, mice were divided into four groups: vehicle (PBS), IR alone (2 Gy/day), CKD-516 (3 mg/kg) alone, and CKD-516 (3 mg/kg, day 1) combined with IR. IR was administered at 2 Gy every 3 weeks for 5 days per cycle and CKD-516 was administered via intraperitoneal injection 1 h after IR on the first day of the cycle (arrow: CKD-516 administration). At the end of each cycle, no treatment was administered for 2 days. Mice were sacrificed 72 h (22 days) after the end of the administration schedule. c CD31 was used to stain tumor tissues using IHC. The number of blood vessels was measured for each group. d The area of tumor necrosis was analyzed by H&E staining (n = 10 per group). The data are presented as the mean ± SE. * denotes p < 0.05, ** denotes p < 0.01, and *** denotes p < 0.001