Fig. 3

Induction of T cell activation by MIL-38-CD3 BiTE. T cell activation was measured by flow cytometry analysis of CD69 (a) or CD25 (c) in T cells (within PBMCs) cultured with MIL-38-CD3 BiTE and GPC-1^high DU-145 cells within the CD3⁺CD4⁺ or CD3⁺CD8⁺ T cell gates (a) or GPC-1^low C3 cells (b: CD69; d: CD25) at a ratio of 10:1 PBMC:tumour cell for 24 h. Data are representative of more than 2 independent experiments. ****p < 0.0001; ***p < 0.001; **p < 0.01