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Fig. 3 | BMC Cancer

Fig. 3

From: The DLC-1 tumor suppressor is involved in regulating immunomodulation of human mesenchymal stromal /stem cells through interacting with the Notch1 protein

Fig. 3

DLC-1 inhibits the immunomodulation of hUC-MSCs. A The DLC-1-silencing in hUC-MSCs, confirmed by Western blotting after siDLC-1 transfection (A-1), enhanced the inhibitory effect of hUC-MSCs with or without GSI-1 on Th1 lymphocyte proliferation, as tested via flow cytometry in Th1 lymphocyte proliferation assay (A-2). The original spectrogram of a representative Th1 lymphocyte analysis is presented (A-3). The Th1 lymphocytes are circled as the CD8/IFN-γ+ cells in the spectrogram. All spectrograms are arranged in alphabetical order corresponding to the groups in A-2. B The DLC-1 overexpression from DLC-1 cDNA transfection in hUC-MSCs, confirmed by Western blotting (B-1), blunted the inhibitory effect of hUC-MSCs on Th1 proliferation, as tested via flow cytometry in the Th1 proliferation assay (B-2). PIB stands for PMA, Ionomycin and Brefeidin. The representative spectrogram of Th1 testing is presented (B-3). The CD8/IFN-γ+ cells as circled in the spectrogram from the CD3-gated lymphocytes were recognized as Th1 lymphocytes. All spectrograms are arranged in alphabetical order corresponding to the groups in B-2. C The co-transfection of siDLC-1 with pIDO1-Luc in hUC-MSCs showed that DLC-1 silencing enhanced the IFN-γ-induced IDO-1 promoter activity. D The co-transfection of DLC-1 cDNA with pIDO1-Luc in hUC-MSCs indicated that DLC-1 over-expression inhibited the IDO1 promoter activity

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