Fig. 9From: Determining the effects of trastuzumab, cetuximab and afatinib by phosphoprotein, gene expression and phenotypic analysis in gastric cancer cell linesEffect of HBEGF knockdown on motility and average speed. MKN1 cells were transfected with negative-control (Ctr) or HBEGF (HBEGF KD) siRNA. The knockdown was checked on RNA level on day 1 after transfection (a). Time-lapse microscopy was carried out for 7âh. Cell movement was tracked to assess motility (b) and average speed (manual) (c). Shown are the mean values of four experiments with standard deviation. Statistically significant effects are indicated by *p <â0.05 (two-sample t-test) (b, c)Back to article page